Activated Microglia in the Early Stage of a Rat Model of Parkinson’s Disease: Revealed by PET-MRI Imaging by [18F]DPA-714 Targeting TSPO

Peizhen Ye, Lei Bi, Min Yang, Yifan Qiu, Guolong Huang, Yongshan Liu, Yuyi Hou, Zhijun Li, Henry Hoi Yee Tong, Mengchao Cui, Hongjun Jin

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Abstract

In the past decades, translocator protein (TSPO) has been considered as an in vivo biomarker to measure the presence of neuroinflammatory reactions. In this study, expression of TSPO was quantified via [18F]DPA-714 positron emission tomography-magnetic resonance imaging (PET-MRI) to investigate the effects of microglial activation associated with motor behavioral impairments in the 6-hydroxydopamine (6-OHDA)-treated rodent model of Parkinson’s disease (PD). [18F]FDG PET-MRI (for non-specific inflammation), [18F]D6-FP-(+)-DTBZ PET-MRI (for damaged dopaminergic (DA) neurons), post-PET immunofluorescence, and Pearson’s correlation analyses were also performed. The time course of striatal [18F]DPA-714 binding ratio was elevated in 6-OHDA-treated rats during 1-3 weeks post-treatments, with peak TSPO binding in the 1st week. No difference between the bilateral striatum in [18F]FDG PET imaging were found. Moreover, an obvious correlation between [18F]DPA-714 SUVRR/L and rotation numbers was found (r = 0.434, *p = 0.049). No correlation between [18F]FDG SUVRR/L and rotation behavior was found. [18F]DPA-714 appeared to be a potential PET tracer for imaging the microglia-mediated neuroinflammation in the early stage of PD.

Original languageEnglish
Pages (from-to)2183-2192
Number of pages10
JournalACS Chemical Neuroscience
Volume14
Issue number11
DOIs
Publication statusPublished - 7 Jun 2023
Externally publishedYes

Keywords

  • Parkinson’s disease
  • TSPO
  • [F]DPA-714
  • microglia
  • positron emission tomography

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