Adventitious root culture of Salvia miltiorrhiza (I) - Effects of various media, salts intensity, and organic components on adventitious root culture of Salvia miltiorrhiza

Objective: To study the effects of various media, salt intensity, and organic components on the growth of Salvia miltiorrhiza adventitious roots and the synthesis of tanshinone II_A and protocatechuic aldehyde. Methods: The adventitious roots were obtained through tissue culture by manipulation of various media, salt intensity, and organic components and the contents of tanshinone II_A and protocatechuic aldehyde were determined by HPLC. Results: The effect of media MS, LS, B₅, White, and SH on adventitious roots of S. miltiorrhiza was observed. Adventitious roots grew better under high salt intensity while secondary metabolite biosynthesis was accelerated under low salt intensity in MS basal medium. The reciprocity of five organic components had significant effect on root growth; glycin favored the synthesis of tanshinone II_A; scarcity of one of inosital, glycin, VB₁, and VB₆ inhibited the synthesis of protocatechuic aldehyde. Conclusion: MS Basal medium is used for adventitious root culture. The results show that salt intensity and organic components have significant effects on adventitious root culture of S. miltiorrhiza and secondary melabolite synthesis.