[Determination of reactive oxygen species in mitochondria by fluorometric probe].

Jin Lei Nie, Qing De Shi, Yong Zhang, Xiao Ming Li, Shu Sen Liu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


AIM AND METHODS: Based on the reaction that 2',7'-dichlorodihydrofluorescein (DCFH) can be oxidized by reactive oxygen species (ROS) to yield the highly fluorescent 2',7'-dichlorofluorescin (DCF), ROS production in mitochondria can be observed dynamically as well as quantified directly by spectrofluorometer. RESULTS AND CONCLUSION: DCF fluorescence showed linear increase in State 4 mitochondria, which suggest ROS produced at constant rate. The slopes of the linear increase in fluorescence with time were computed performing a linear regression that took into account all relevant data points in selected time windows. The slopes were proportional to ROS production in mitochondria. Addition of sodium azide and malonic acid increased and decreased the rate of ROS production respectively during measurement. DCF fluorescence varied linearly with increasing concentration of mitochondria, which showed quantitative relations in definite range. Repeated measures showed low coefficients of variation. This method is reliable and efficient for determining ROS in mitochondria.

Original languageEnglish
Pages (from-to)196-198
Number of pages3
JournalZhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Issue number2
Publication statusPublished - May 2002


Dive into the research topics of '[Determination of reactive oxygen species in mitochondria by fluorometric probe].'. Together they form a unique fingerprint.

Cite this