Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats

Qing Huang, Lan Shao, Hong Jiang, Zhen Chuan Miao, Qing De Shi, Shu Sen Liu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


AIM: To observe the effects of insulin on liver mitochondrial respiratory function, activity of H+-ATPase, and superoxide anion free radicals production in diabetic rats. METHODS: Rats were injected iv with alloxan 40 mg/kg to induce diabetes. The liver mitochondrial respiratory function was assayed by measurement of oxygen consumption using a Clark oxygen electrode. Superoxide anion production was assayed using chemiluminescence method. Activities of H+-ATPase were measured by luciferin-luciferase system and inorganic phosphorus's method. RESULTS: Insulin 1 U/kg sc daily for 9 weeks improved oxidative phosphorylation, respiratory rate state 3 (P < 0.05), respiratory control ration (P < 0.01), and ADP:O ratio (P <0.01), but there were no obvious effect on respiratory rate state 4 (P > 0.05). In the insulin group, synthesis activity of H+-ATPase was obviously increased (P < 0.05) and hydrolytic activity of H+-ATPase was remarkably decreased (P < 0.01), compared with the diabetes group. Insulin 1 U/kg for 9 weeks apparently decreased the production of O2-(P < 0. 01) in liver mitochondria of diabetic rats. CONCLUSION: Insulin can prevent the injury from superoxide anion in liver mitochondria, and improve the function of the liver mitochondria oxidative phosphorylation.

Original languageEnglish
Pages (from-to)455-458
Number of pages4
JournalActa Pharmacologica Sinica
Issue number5
Publication statusPublished - 2001
Externally publishedYes


  • Experimental diabetes mellitus
  • Free radicals
  • Insulin
  • Mitochondria
  • Oxidative phosphorylation


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