TY - JOUR
T1 - Fluorimetric investigation of supramolecular system by modified β-cyclodextrin and its analytical application
AU - Huang, Lizhen
AU - He, Jiang
AU - Ge, Xia
AU - Lu, Ruihua
AU - Guo, Jingjing
N1 - Funding Information:
The work is supported by the National Natural Science Foundation of China (No. J0730425 ) and the Main Nature Science Foundation of Gansu Province in China (No. 3ZS041-A25-009 ).
PY - 2011/5
Y1 - 2011/5
N2 - The supramolecular interaction of MAH-β-cyclodextrin (MAH-β-CD, a modified β-cyclodextrin carrying seven vinyl carboxylic acid groups) and meferamic acid (MF) has been studied by spectrofluorimetry. The results showed that MAH-β-CD reacted with MF to form a host-guest complex (MAH-β-CD-MF) with stoichiometry (1:1) and the inclusion constant (K = 7.15 × 102 L/mol) was ascertained by the typical double reciprocal plots. From the phase-solubility diagram, an increase in the water solubility of the drug was observed and the apparent stability constant (K1:1) was calculated to be 8.62 × 102 L/mol. Furthermore, the thermodynamic parameters (ΔG°, ΔH° and ΔS°) of MAH-β-CD-MF were obtained and the inclusion mechanism was also preliminarily discussed. In order to further confirm the experimental results, investigation on the molecular modeling was performed. On the basis of the significant enhancement of the fluorescence intensity of MF, a spectrofluorimetric method for MF determination in bulk aqueous solution in the presence of MAH-β-CD was developed. The linear range was 2.00 × 10-8-9.00 × 10-5 mol/L and the detection limit was 3.36 × 10-9 mol/L. The proposed method was successfully applied to determine MF in tablets, serum and urine with the satisfactory result.
AB - The supramolecular interaction of MAH-β-cyclodextrin (MAH-β-CD, a modified β-cyclodextrin carrying seven vinyl carboxylic acid groups) and meferamic acid (MF) has been studied by spectrofluorimetry. The results showed that MAH-β-CD reacted with MF to form a host-guest complex (MAH-β-CD-MF) with stoichiometry (1:1) and the inclusion constant (K = 7.15 × 102 L/mol) was ascertained by the typical double reciprocal plots. From the phase-solubility diagram, an increase in the water solubility of the drug was observed and the apparent stability constant (K1:1) was calculated to be 8.62 × 102 L/mol. Furthermore, the thermodynamic parameters (ΔG°, ΔH° and ΔS°) of MAH-β-CD-MF were obtained and the inclusion mechanism was also preliminarily discussed. In order to further confirm the experimental results, investigation on the molecular modeling was performed. On the basis of the significant enhancement of the fluorescence intensity of MF, a spectrofluorimetric method for MF determination in bulk aqueous solution in the presence of MAH-β-CD was developed. The linear range was 2.00 × 10-8-9.00 × 10-5 mol/L and the detection limit was 3.36 × 10-9 mol/L. The proposed method was successfully applied to determine MF in tablets, serum and urine with the satisfactory result.
KW - Analytical application
KW - Mefenamic acid
KW - Modified β-cyclodextrin
KW - Molecular modeling
KW - Spectrofluorimetry
UR - http://www.scopus.com/inward/record.url?scp=79953024702&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2011.01.049
DO - 10.1016/j.saa.2011.01.049
M3 - Article
C2 - 21339082
AN - SCOPUS:79953024702
SN - 1386-1425
VL - 78
SP - 1553
EP - 1559
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
IS - 5
ER -