In silico and in vitro anti-Helicobacter pylori effects of combinations of phytochemicals and antibiotics

Pedro Fong, Chon Hou Hao, Chi Cheng Io, Pou Io Sin, Li Rong Meng

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Helicobacter pylori infection is a WHO class 1 carcinogenic factor of gastric adenocarcinoma. In the past decades, many studies have demonstrated the increasing trend of antibiotic resistance and pointed out the necessity of new effective treatment. This study was aimed at identifying phytochemicals that can inhibit H. pylori and possibly serve as adjuvant treatments. Here, in silico molecular docking and drug-like properties analyses were performed to identify potential inhibitors of urease, shikimate kinase and aspartate-semialdehyde dehydrogenase. These three enzymes are targets of the treatment of H. pylori. Susceptibility and synergistic testing were performed on the selected phytochemicals and the positive control antibiotic, amoxicillin. The in-silico study revealed that oroxindin, rosmarinic acid and verbascoside are inhibitors of urease, shikimate kinase and aspartate-semialdehyde dehydrogenase, respectively, in which, oroxindin has the highest potency against H. pylori, indicated by a minimum inhibitory concentration (MIC) value of 50 µg/mL. A combination of oroxindin and amoxicillin demonstrated additive effects against H. pylori, as indicated by a fractional inhibitory concentration (FIC) value of 0.75. This study identified phytochemicals that deserve further investigation for the development of adjuvant therapeutic agents to current antibiotics against H. pylori.

Original languageEnglish
Article number3608
JournalMolecules
Volume24
Issue number19
DOIs
Publication statusPublished - 7 Oct 2019

Keywords

  • Antibacterial phytochemicals
  • Antibiotic resistance
  • Bacterial protein targets
  • Helicobacter pylori inhibition
  • Molecular docking

Fingerprint

Dive into the research topics of 'In silico and in vitro anti-Helicobacter pylori effects of combinations of phytochemicals and antibiotics'. Together they form a unique fingerprint.

Cite this