Intestinal absorption of puerarin nanocrystalline self-stabilizing pickering emulsion in rats

To study the intestinal absorption characteristics of drug's nanocrystalline self-stabilizing Pickering emulsion (NSSPE) in situ in rats. Rat single-pass intestinal perfusion model was established, and high performance liquid chromatography (HPLC) was used to detect the concentration of puerarin in rat intestinal perfusion solution, assay the absorption rate constant (K _a ) and the intestinal apparent permeability coefficient (P _app ) of NSSPE in duodenum, jejunum, ileum, and colon, which were compared with those of raw material, nanocrystal and normal emulsion, respectively. For NSSPE, the K _a and P _app values were in the following order: duodenum>jejunum>ileum (P<0.05) >colon (P<0.01). However, there was no obvious difference between jejunum and ileum. As compared with raw material, nanocrystal and normal emulsion, the K _a and P _app values of NSSPE in duodenum were significantly higher than those of other three preparations (P<0.05); and the K _a and P _app values of NSSPE in jejunum and colon were significantly higher than those of raw material, nanocrystal and normal emulsion (P<0.01); and the K _a and P _app of NSSPE in ileum were also higher than those of raw material and normal emulsion (P<0.05), but had no obvious difference with nanocrystal. The results showed that NSSPE could significantly improve the absorption of puerarin in the intestine of rats.