Microemulsion electrokinetic chromatography with laser-induced fluorescence detection: As tested with amino acid derivatives

Xie Jianping, Zhang Jiyou, Liu Huanxiang, Liu Jiaqin, Tian Jianniao, Chen Xingguo, Hu Zhide

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Over a decade ago, microemulsion electrokinetic chromatography was introduced as a novel mode of capillary electrophoresis. However, there has not been publication on the combination of microemulsion electrokinetic chromatography with laser-induced fluorescence detection. In this paper, a preliminary method using microemulsion eletrokinetic chromatography combined with laser-induced fluorescence detection and second derivative electrophoregram was established as a sensitive and selective assay for separation and determination of nine amino acids after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. The derivatization and separation conditions were optimized. In the investigated concentration ranges correlation coefficients were better than 0.995. The relative standard deviation (n = 5) of the migration times and peak heights were 0.56-0.76 and 2.21-7.15%, respectively. The detection limits (S/N = 3) were at a neaomolar level (0.32-2.20 nM). The method was applied for the analysis of compound amino acid injection and a Chinese traditional herbal medicine. The recoveries were 95.9-107.9%.

Original languageEnglish
Pages (from-to)600-607
Number of pages8
JournalBiomedical Chromatography
Volume18
Issue number8
DOIs
Publication statusPublished - Oct 2004
Externally publishedYes

Keywords

  • Amino acids
  • Laser-induced fluorescence
  • Microemulsion electrokinetic chromatography
  • Multiple linear regression
  • Second derivative electrophoregram

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