miR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells

Bin Fu, Yibing Wang, Xiali Zhang, Bin Lang, Xiaocheng Zhou, Xiaoyuan Xu, Tao Zeng, Weipeng Liu, Xu Zhang, Ju Guo, Gongxian Wang

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR-221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR-221. As a result, the repression of miR-221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells.

Original languageEnglish
Pages (from-to)1169-1180
Number of pages12
JournalInternational Journal of Oncology
Volume46
Issue number3
DOIs
Publication statusPublished - 1 Mar 2015

Keywords

  • Bladder cancer
  • Immune escape
  • miR-221

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