Novel RGD-containing peptides exhibited improved abilities to integrin receptor binding and cultures of human induced pluripotent stem cells

Ping Zhou, Fang Feng, Yameng Song, Jing Li, Qin Li, Zerong Xu, Jiamin Shi, Liying Qin, Fei He, Hongjiao Li, Yu Han, Rongzhi Zhang, Huanxiang Liu, Feng Lan

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The design of novel peptide sequences and investigations of the mechanisms underlying cell adhesion is critical for the culture of human induced pluripotent stem cells (hiPSCs) on the peptide displaying surfaces. Recently, we reported that near-Asp sequences play an important role in the function of whole Arg-Gly-Asp (RGD)-containing peptides. In this study, two novel peptides with sequences of Ac-KGGTYRAYRGDVFTMP and Ac-KGGVFTMPRGDTYRAY were designed from reported peptides. Fortunately, the Ac-KGGVFTMPRGDTYRAY peptide exhibited excellent ability in sustaining cultures of hiPSCs. Moreover, we predicted the structural model of peptides by molecular dynamics simulation and successfully obtained the complex structure of peptides and αVβ3/αVβ5 integrin proteins through molecular docking. Finally, a strong affinity to the αVβ3 integrin receptor contributes to the excellent ability of peptide was confirmed. Study reveals the mechanisms by which RGD-containing peptides support the adhesion and provides a better peptide for hiPSCs culture.

Original languageEnglish
Article number110762
JournalMaterials and Design
Volume219
DOIs
Publication statusPublished - Jul 2022
Externally publishedYes

Keywords

  • Human induced pluripotent stem cells
  • Integrin receptor
  • Peptides
  • RGD sequence
  • Synthetic substrate

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