Over-expression of LRIG3 suppresses growth and invasion of bladder cancer cells

Yong Qi, Lei Chang, Heng Li, Gan Yu, Wei Xiao, Ding Xia, Wei Guan, Yang Yang, Bin Lang, Kang Li Deng, Wei Min Yao, Zhang Qun Ye, Qian Yuan Zhuang

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The purpose of this study was to investigate the impact of leucine-rich repeats and immunoglobulin-like domains 3 (LRIG3) on the biological features of bladder cancer cell lines. The plasmids of over-expressed LRIG3 and the blank plasmid serving as control were transfected into the bladder cancer cell lines, T24, EJ and BIU-87, and the expression levels of LRIG3 mRNA and protein were detected by using real-time PCR and Western blotting. The changes in the cell cycle and apoptosis were examined by using flow cytometry. The invasive ability was measured by Transwell assay, and CCK-8 assays were used to measure the proliferation of cells. As compared with the control group, the LRIG3 mRNA and protein expression levels in LRIG3 cDNA-transfected group were raised significantly (P<0.05). The average number of cells with up-regulated LRIG3 passing through the inserted filter was decreased significantly as compared with the control group (P<0.05). Up-regulation of LRIG3 also could inhibit proliferation and induce apoptosis of T24, EJ and BIU-87 cells. Except BIU-87, the T24 and EJ cells transfected with LIRG3 cDNA were arrested in G 0/G1 phase compared to the control group (P<0.05). In conclusion, the over-expression of LRIG3 could influence the cell cycle and invasion, inhibit proliferation and induce apoptosis in the three bladder cancer cell lines.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalJournal of Huazhong University of Science and Technology - Medical Science
Issue number1
Publication statusPublished - Feb 2013


  • LRIG3
  • apoptosis
  • bladder cancer cell lines
  • cell cycle
  • invasion


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