TY - JOUR
T1 - Preparation and characterization of monoclonal antibodies against avermectin
AU - Shiwen, Xu
AU - Shu, Li
AU - Dongbo, Sun
AU - Donghua, Guo
PY - 2009/6/1
Y1 - 2009/6/1
N2 - The coupled antigen, avermectin (AVM) and bovine serum albumin (BSA), was artificially synthesized. Female 8-week-old BALB/c mice were immunized with AVM-BSA emulsified in Freund's adjuvant. Two monoclonal antibodies (MAbs) against AVM, named 2F2 and 2H10, were prepared by lymphocyte hybridoma technique. Ascitic fluid titers of the MAbs 2F2 and 2H10 were, respectively, 1:6400 and 1:25, 600, and their subtypes were all IgMI. In competitive indirect ELISA (Ci-ELISA), 50% inhibiting concentration (IC50) of the MAb 2H10 for avermectin was 101/ng/mL. In specific assay, the MAb 2H10 could not react with ivermectin, doramectin, erythromycin, and albomycin. These data demonstrated that the MAb 2H10 will have a potential use for developing diagnostic reagents of avermectin residues.
AB - The coupled antigen, avermectin (AVM) and bovine serum albumin (BSA), was artificially synthesized. Female 8-week-old BALB/c mice were immunized with AVM-BSA emulsified in Freund's adjuvant. Two monoclonal antibodies (MAbs) against AVM, named 2F2 and 2H10, were prepared by lymphocyte hybridoma technique. Ascitic fluid titers of the MAbs 2F2 and 2H10 were, respectively, 1:6400 and 1:25, 600, and their subtypes were all IgMI. In competitive indirect ELISA (Ci-ELISA), 50% inhibiting concentration (IC50) of the MAb 2H10 for avermectin was 101/ng/mL. In specific assay, the MAb 2H10 could not react with ivermectin, doramectin, erythromycin, and albomycin. These data demonstrated that the MAb 2H10 will have a potential use for developing diagnostic reagents of avermectin residues.
UR - http://www.scopus.com/inward/record.url?scp=67651165140&partnerID=8YFLogxK
U2 - 10.1089/hyb.2009.0002
DO - 10.1089/hyb.2009.0002
M3 - Article
C2 - 19519244
AN - SCOPUS:67651165140
SN - 1554-0014
VL - 28
SP - 173
EP - 176
JO - Hybridoma
JF - Hybridoma
IS - 3
ER -