Recent advances in CE-mediated microanalysis for enzyme study

Xu Wang, Kefeng Li, Erwin Adams, Ann Van Schepdael

Research output: Contribution to journalReview articlepeer-review

47 Citations (Scopus)

Abstract

This review gives an overview of the recent developments and applications in the use of CE-mediated microanalysis for enzyme studies. The period covers mid-2011 until mid-2013. Both off-line and in-line enzyme assays with their applications using CE are described in this article. For the in-capillary enzyme reaction, the techniques using electrophoretically mediated microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme-mediated drug metabolism.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalElectrophoresis
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

Keywords

  • CE
  • Electrophoretically mediated microanalysis
  • Enzyme study
  • In-line
  • Off-line

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