Unveiling the Mechanism of Phenamacril Resistance in F. graminearum: Computational and Experimental Insights into the C423A Mutation in FgMyoI

Yiqiong Bao, Fangying Jia, Yun Lin, Guohong Song, Mengrong Li, Ran Xu, Hancheng Wang, Feng Zhang, Jingjing Guo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Phenamacril (PHA) is a highly selective fungicide for controlling fusarium head blight (FHB) mainly caused by F. graminearum and F. asiaticum. However, the C423A mutation in myosin I of F. graminearum (FgMyoI) leads to natural resistance to PHA. Here, based on the computational approaches and biochemical validation, we elucidate the atomic-level mechanism behind the natural resistance of F. graminearum to the fungicide PHA due to the C423A mutation in FgMyoI. The mutation leads to a rearrangement of pocket residues, resulting in increased size and flexibility of the binding pocket, which impairs the stable binding of PHA. MST experiments confirm that the mutant protein FgMyoIC423A exhibits significantly reduced affinity for PHA compared to wild-type FgMyoI and the nonresistant C423K mutant. This decreased binding affinity likely underlies the development of PHA resistance in F. graminearum. Conversely, the nonresistant C423K mutant retains sensitivity to PHA due to the introduction of a strong hydrogen bond donor, which facilitates stable binding of PHA in the pocket. These findings shed light on the molecular basis of PHA resistance and provide new directions for the creation of new myosin inhibitors.

Original languageEnglish
Pages (from-to)15653-15661
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume72
Issue number28
DOIs
Publication statusPublished - 17 Jul 2024

Keywords

  • conformational response
  • myosin
  • phenamacril
  • pocket perturbation
  • resistant-mutation

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