Effects of interference of XIAP by siRNA on the biological behaviors in ovarian carcinoma SKOV-3 cells

OBJECTIVE: To silence the expression of XIAP in ovarian cancer cell line SKOV-3 by using small interfering RNA (siRNA), and observe its effects on the proliferation and apoptosis. METHODS: The XIAP siRNA was transfected into SKOV-3 cells and the cell proliferation was evaluated using MTT assay. The apoptosis rate and cell cycle distribution were analyzed using Annexin-V and flow cytometry methods. RESULTS: XIAP siRNA significantly suppressed the expression of XIAP gene in SKOV-3 cells. The difference was significant compared with other groups (F=63. 782, P<0. 05). Specific group cells were slower than normal and nonspecific groups in the rate of cell proliferation (F=53. 412, P<0. 05). The apoptosis percentage of interference group [(31. 54±0. 62)%] was higher than that of nonspecific [(5. 34 ±0. 38)%] and control groups [(6. 73±0. 46)%, (F=102. 32, P<0. 01)]. After transfection XIAP-siRNA, SKOV-3 cells were arrested at G₀/G₁ phase. The difference was significant compared with other groups (P<0. 01). CONCLUSION: Silencing XIAP gene can inhibite the proliferation and induced the apoptosis of SKOV-3 cells.