TY - JOUR
T1 - FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity
AU - Cui, Zifeng
AU - Tian, Rui
AU - Huang, Zhaoyue
AU - Jin, Zhuang
AU - Li, Lifang
AU - Liu, Jiashuo
AU - Huang, Zheying
AU - Xie, Hongxian
AU - Liu, Dan
AU - Mo, Haiyan
AU - Zhou, Rong
AU - Lang, Bin
AU - Meng, Bo
AU - Weng, Haiyan
AU - Hu, Zheng
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Genome editing technologies hold tremendous potential in biomedical research and drug development. Therefore, it is imperative to discover gene editing tools with superior cutting efficiency, good fidelity, and fewer genomic restrictions. Here, we report a CRISPR/Cas9 from Faecalibaculum rodentium, which is characterized by a simple PAM (5′-NNTA-3′) and a guide RNA length of 21–22 bp. We find that FrCas9 could achieve comparable efficiency and specificity to SpCas9. Interestingly, the PAM of FrCas9 presents a palindromic sequence, which greatly expands its targeting scope. Due to the PAM sequence, FrCas9 possesses double editing-windows for base editor and could directly target the TATA-box in eukaryotic promoters for TATA-box related diseases. Together, our results broaden the understanding of CRISPR/Cas-mediated genome engineering and establish FrCas9 as a safe and efficient platform for wide applications in research, biotechnology and therapeutics.
AB - Genome editing technologies hold tremendous potential in biomedical research and drug development. Therefore, it is imperative to discover gene editing tools with superior cutting efficiency, good fidelity, and fewer genomic restrictions. Here, we report a CRISPR/Cas9 from Faecalibaculum rodentium, which is characterized by a simple PAM (5′-NNTA-3′) and a guide RNA length of 21–22 bp. We find that FrCas9 could achieve comparable efficiency and specificity to SpCas9. Interestingly, the PAM of FrCas9 presents a palindromic sequence, which greatly expands its targeting scope. Due to the PAM sequence, FrCas9 possesses double editing-windows for base editor and could directly target the TATA-box in eukaryotic promoters for TATA-box related diseases. Together, our results broaden the understanding of CRISPR/Cas-mediated genome engineering and establish FrCas9 as a safe and efficient platform for wide applications in research, biotechnology and therapeutics.
UR - http://www.scopus.com/inward/record.url?scp=85126547301&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-29089-8
DO - 10.1038/s41467-022-29089-8
M3 - Article
C2 - 35301321
AN - SCOPUS:85126547301
SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1425
ER -