Influence of KCC1 gene regulate ERK pathway to invasion ability of endometrial cancer cells

OBJECTIVE: To explore the mechanism of KCC1 regulates the invasion ability of endometrial cancer HEC-1B cells through the extracellular signal-regulated kinase (ERK) signaling pathway. METHODS: The expression of KCC1 and phosphorylated ERK1/2 proteins and invasion ability in the HEC-1B cells after pcDNA-KCC1 transfection were detected by western blot and cell invasion assay. After treatment with U0126, the quantity of p-ERK1/2 and the cell invasion ability were measured again. RESULTS: After pcDNA-KCC1 transfected to the HEC-1B cells, the expression of KCC1 and p-ERK1/2 increased dramatically (P<0.05), and the number of cells penetrated filter membrane increased from 26.7±2.3 to 50.3±3.1 (P<0.05). In HEC-1B cells treated by pcDNA-KCCl transfection, treatment of U0126 could inhibit phosphorylation of ERK1/2 protein (P<0.05), and the number of cells penetrated filter membrane decreased from 50.3±3.1 to 30.8±5.9 (P<0.05). CONCLUSION: KCC1 gene may be participates in the invasion ability of HEC-1B cells through the ERK signaling pathway.