TY - JOUR
T1 - Intestinal absorption of puerarin nanocrystalline self-stabilizing pickering emulsion in rats
AU - Wang, Shuai
AU - Li, Qing Qing
AU - Wang, Fan
AU - Yi, Tao
AU - Zhang, Ji Fen
N1 - Publisher Copyright:
© 2018, Editorial Board of China Journal of Chinese Materia Medica. All right reserved.
PY - 2018/5/15
Y1 - 2018/5/15
N2 - To study the intestinal absorption characteristics of drug's nanocrystalline self-stabilizing Pickering emulsion (NSSPE) in situ in rats. Rat single-pass intestinal perfusion model was established, and high performance liquid chromatography (HPLC) was used to detect the concentration of puerarin in rat intestinal perfusion solution, assay the absorption rate constant (K a ) and the intestinal apparent permeability coefficient (P app ) of NSSPE in duodenum, jejunum, ileum, and colon, which were compared with those of raw material, nanocrystal and normal emulsion, respectively. For NSSPE, the K a and P app values were in the following order: duodenum>jejunum>ileum (P<0.05) >colon (P<0.01). However, there was no obvious difference between jejunum and ileum. As compared with raw material, nanocrystal and normal emulsion, the K a and P app values of NSSPE in duodenum were significantly higher than those of other three preparations (P<0.05); and the K a and P app values of NSSPE in jejunum and colon were significantly higher than those of raw material, nanocrystal and normal emulsion (P<0.01); and the K a and P app of NSSPE in ileum were also higher than those of raw material and normal emulsion (P<0.05), but had no obvious difference with nanocrystal. The results showed that NSSPE could significantly improve the absorption of puerarin in the intestine of rats.
AB - To study the intestinal absorption characteristics of drug's nanocrystalline self-stabilizing Pickering emulsion (NSSPE) in situ in rats. Rat single-pass intestinal perfusion model was established, and high performance liquid chromatography (HPLC) was used to detect the concentration of puerarin in rat intestinal perfusion solution, assay the absorption rate constant (K a ) and the intestinal apparent permeability coefficient (P app ) of NSSPE in duodenum, jejunum, ileum, and colon, which were compared with those of raw material, nanocrystal and normal emulsion, respectively. For NSSPE, the K a and P app values were in the following order: duodenum>jejunum>ileum (P<0.05) >colon (P<0.01). However, there was no obvious difference between jejunum and ileum. As compared with raw material, nanocrystal and normal emulsion, the K a and P app values of NSSPE in duodenum were significantly higher than those of other three preparations (P<0.05); and the K a and P app values of NSSPE in jejunum and colon were significantly higher than those of raw material, nanocrystal and normal emulsion (P<0.01); and the K a and P app of NSSPE in ileum were also higher than those of raw material and normal emulsion (P<0.05), but had no obvious difference with nanocrystal. The results showed that NSSPE could significantly improve the absorption of puerarin in the intestine of rats.
KW - Intestinal absorption
KW - Nanocrystalline
KW - Pickering emulsion
KW - Puerarin
KW - Single-pass intestinal perfusion
UR - http://www.scopus.com/inward/record.url?scp=85049317557&partnerID=8YFLogxK
M3 - Article
C2 - 29933687
AN - SCOPUS:85049317557
SN - 1001-5302
VL - 43
SP - 2162
EP - 2167
JO - Zhongguo Zhongyao Zazhi
JF - Zhongguo Zhongyao Zazhi
IS - 10
ER -