MiRNA-34a suppresses cell proliferation and metastasis by targeting CD44 in human renal carcinoma cells

Gan Yu, Heng Li, Ji Wang, Kiranmai Gumireddy, Anping Li, Weimin Yao, Kun Tang, Wei Xiao, Junhui Hu, Haibing Xiao, Bin Lang, Zhangqun Ye, Qihong Huang, Hua Xu

研究成果: Article同行評審

68 引文 斯高帕斯(Scopus)

摘要

Purpose We investigated the potential functions of miR-34a in CD44 transcriptional complexes in renal cell carcinoma. Materials and Methods We detected miR-34a expression by quantitative real-time polymerase chain reaction. Oligonucleotides were used to over express miR-34a. Cell proliferation and xenograft assays, colony formation and flow cytometry were done to examine effects on cancer cell proliferation in vitro and in vivo. Luciferase assay was performed to verify the precise target of miR-34a. Results Promoter methylation contributed to miR-34a loss in the ACHN, 786-O and SN12PM6 renal carcinoma cell lines. Ectopic over expression of miR-34a restrained cell growth, tube formation and migration/invasion, and significantly suppressed the growth of renal carcinoma xenografts and metastasis in nude mice. Dual luciferase assay revealed that CD44 was a direct target of miR-34a in renal cancer cells and CD44 knockdown by RNAi in renal cancer cells suppressed tumor progression. In contrast, CD44 ectopic expression partially reversed the antitumor effects of miR-34a in renal cancer cells. Conclusions Our findings indicate that miR-34a targets CD44 in renal cancer cells and suppresses renal cancer cell growth, tube formation and metastasis in vitro and in vivo. Thus, miR-34a may be a potential molecular target for novel therapeutic strategies for clear cell renal carcinoma.

原文English
頁(從 - 到)1229-1237
頁數9
期刊Journal of Urology
192
發行號4
DOIs
出版狀態Published - 1 10月 2014

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