Preparation and characterization of monoclonal antibodies against avermectin

The coupled antigen, avermectin (AVM) and bovine serum albumin (BSA), was artificially synthesized. Female 8-week-old BALB/c mice were immunized with AVM-BSA emulsified in Freund's adjuvant. Two monoclonal antibodies (MAbs) against AVM, named 2F2 and 2H10, were prepared by lymphocyte hybridoma technique. Ascitic fluid titers of the MAbs 2F2 and 2H10 were, respectively, 1:6400 and 1:25, 600, and their subtypes were all IgMI. In competitive indirect ELISA (Ci-ELISA), 50% inhibiting concentration (IC₅₀) of the MAb 2H10 for avermectin was 101/ng/mL. In specific assay, the MAb 2H10 could not react with ivermectin, doramectin, erythromycin, and albomycin. These data demonstrated that the MAb 2H10 will have a potential use for developing diagnostic reagents of avermectin residues.